UV photobiochemistry of anhydrobiotic organisms at extremely low temperatures

Spores of Bacillus subtilis (TKJ 3412), cells of Deinococcus radiodurans R₁ (wild type) and conidia of Aspergillus ochraceus (strain 3174) have been UV irradiated (254 nm) in the dry state (3% relative humidity, argon) or in aqueous suspension at room temperature, at −55°C to −70°C and at −165°C to −170°C. The following effects have been analyzed: decrease in viability, occurrence of DNA strand breaks (pulsed-field gel electrophoresis) and production of DNA-protein cross-links (membrane filter method). The loss in viability is usually more pronounced at around −70°C than at room temperature, but it is lowest around −170°C. The kind of prevailing DNA damage varies from organism to organism. The amount of UV induced DNA-protein cross-link products steadily decreases with the temperature and is lowest at −170°C. The decrease in highly polymeric DNA by double strand breaks follows no universal pattern. The observed hypersensitivity of the three very different species at −70°C can therefore not be simply explained on the basis of the number of DNA lesions analyzed in the course of this work. We suggest that also the changing state of cellular water below and above about −130°C significantly contributes to the change in photosensitivity.     

The first dedicated Life Sciences mission--Spacelab 4.

Spacelab is a large versatile laboratory carried in the bay of the Shuttle Orbiter. The first Spacelab mission dedicated entirely to Life Sciences is known as Spacelab 4. It is scheduled for launch in late 1985 and will remain aloft for seven days. This payload consists of 25 tentatively selected investigations combined into a comprehensive integrated exploration of the effects of acute weightlessness on living systems. An emphasis is placed on studying physiological changes that have been previously observed in manned space flight. This payload has complementary designs in the human and animal investigations in order to validate animal models of human physiology in weightlessness. The experimental subjects include humans, squirrel monkeys, laboratory rats, several species of plants, and frog eggs. The primary scientific objectives include study of the acute cephalic fluid shift, cardiovascular adaptation to weightlessness, including postflight reductions in orthostatic tolerance and exercise capacity, and changes in vestibular function, including space motion sickness, associated with weightlessness. Secondary scientific objectives include the study of red cell mass reduction, negative nitrogen balance, altered calcium metabolism, suppressed in vitro lymphocyte reactivity, gravitropism and photropism in plants, and fertilization and early development in frog eggs. The rationale behind this payload, the selection process, and details of the individual investigations are presented in this paper.     

U.S. biological experiments in space.

Past U.S. space biological experiments in space, using non-human specimens, are discussed and evaluated. Current plans for future experimentation in this field are also given.     

Multi-Wavelength Observations of CMEs and Associated Phenomena

This chapter reviews how our knowledge of CMEs and CME-associated phenomena has been improved, since the launch of the SOHO mission, thanks to multi-wavelength analysis. The combination of data obtained from space-based experiments and ground based instruments allows us to follow the space-time development of an event from the bottom of the corona to large distances in the interplanetary medium. Since CMEs originate in the low solar corona, understanding the physical processes that generate them is strongly dependant on coordinated multi-wavelength observations. CMEs display a large diversity in morphology and kinematic properties, but there is presently no statistical evidence that those properties may serve to group them into different classes. When a CME takes place, the coronal magnetic field undergoes restructuring. Much of the current research is focused on understanding how the corona sustains the stresses that allow the magnetic energy to build up and how, later on, this magnetic energy is released during eruptive flares and CMEs. Multi-wavelength observations have confirmed that reconnection plays a key role during the development of CMEs. Frequently, CMEs display a rather simple shape, exhibiting a well known three-part structure (bright leading edge, dark cavity and bright knot). These types of events have led to the proposal of the ‘`standard model’' of the development of a CME, a model which predicts the formation of current sheets. A few recent coronal observations provide some evidence for such sheets. Other more complex events correspond to multiple eruptions taking place on a time scale much shorter than the cadence of coronagraph instruments. They are often associated with large-scale dimming and coronal waves. The exact nature of these waves and the physical link between these different manifestations are not yet elucidated. We also discuss what kind of shocks are produced during a flare or a CME. Several questions remain unanswered. What is the nature of the shocks in the corona (blast-wave or piston-driven?) How they are related to Moreton waves seen in Hα? How they are related to interplanetary shocks? The last section discusses the origin of energetic electrons detected in the corona and in the interplanetary medium. “Complex type III-like events,”which are detected at hectometric wavelengths, high in the corona, and are associated with CMEs, appear to originate from electrons that have been accelerated lower in the corona and not at the bow shock of CMEs. Similarly, impulsive energetic electrons observed in the interplanetary medium are not the exclusive result of electron acceleration at the bow shocks of CMEs; rather they have a coronal origin.     

Energetic Particle Observations

The characteristics of solar energetic particles (SEP) as observed in interplanetary space provide fundamental information about the origin of these particles, and the acceleration and propagation processes at the Sun and in interplanetary space. Furthermore, energetic particles provide information on the development and structure of coronal mass ejections as they propagate from the solar corona into the interplanetary medium. In this paper we review the measurements of energetic particles in interplanetary space and discuss their implication for our understanding of the sources, and of acceleration and propagation processes.     

A Boolean algebra approach to multiple sensor voting fusion

A technique for integrating multiple-sensor data using a voting fusion process that combines the individual sensor outputs is described. An important attribute of the method is the automatic confirmation of the target by the fusion processor without the need to explicitly determine which sensors and what level of sensor participation are involved. A three-sensor system, with multiple confidence levels in each sensor, is discussed to illustrate the approach. Boolean algebra is used to derive closed-form expressions for the multiple sensor-system detection probability and false-alarm probability. Procedures for relating confidence levels to detection and false alarm probabilities are described through an example. The hardware implementation for the sensor system fusion algorithm is discussed     

Calibration of complex polarimetric SAR imagery using backscattercorrelations

A technique for calibration of multipolarization synthetic-aperture radar (SAR) imagery is described. If scatterer reciprocity and lack of correlation between co- and cross-polarized radar echoes (for azimuthally symmetric distributed targets) are assumed, the effects of signal leakage between the radar data channels can be removed without the use of known ground targets. If known targets are available, all data channels can be calibrated relative to one another and radiometrically as well. The method is verified with simulation and application to airborne SAR data     

EVA: European plans

The current status of European EVA (extravehicular activity) plans is reviewed. The major difference to already existing EVA scenarios in U.S.A. and Soviet Union consists in the adoption of a higher suit pressure, namely 500 hPa. The results of a study concerned with the physiological consequences of this adoption are presented, including recommendations for protective procedures and their necessary experimental validation. A certain discrepancy between laboratory experimental decompression data and EVA operational results is discussed, leading to the identification of several items which may influence space decompression. Microgravity and the influence of the space suit itself are most likely factors in the explanation of this discrepancy, and both experimental procedures and technological developments are proposed to clarify their role for the future design of EVA procedures.     

The effects of space flight on some rat liver enzymes regulating carbohydrate and lipid metabolism

We have examined, in the livers of rats carried aboard the Cosmos 936 biosatellite, the activities of about 30 enzymes concerned with carbohydrate and lipid metabolism. In addition to the enzyme studies, the levels of glycogen and of the individual fatty acids in hepatic lipids were determined. Livers from flight and ground control rats at recovery (R₀) and 25 days after recovery (R₂₅) were used for these analyses.

For all parameters measured, the most meaningful comparisons are those made between flight stationary (FS) and flight centrifuged (FC) animals at R₀. When these two groups of flight rats were compared at R₀, statistically significant decreases in the activity levels of glycogen phosphorylase, -glycerol phosphate acyl transferase, diglyceride acyl transferase, aconitase and 6-phosphogluconate dehydrogenase and an increase in the palmitoyl CoA desaturase were noted in the weightless group (FS). The significance of these findings was strengthened by the fact that all enzyme activities showing alterations at R₀ returned to normal 25 days postflight. When liver glycogen and total fatty acids of the two sets of flight animals were determined, significant differences that could be attributed to reduced gravity were observed. The weightless group (FS) at R₀ contained, on the average, more than twice the amount of glycogen than did the centrifuged controls (FC) and a remarkable shift in the ratio of palmitate to palmitoleate was noted. These metabolic alterations, both in enzyme levels and in hepatic constituents, appear to be characteristic of the weightless condition. Our data seem to justify the conclusion that centrifugation during flight is equivalent to terrestrial gravity.